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Estrogen Receptor Antibody [SPM567]

Estrogen Receptor Antibody [SPM567]

€788.00

Excl. VAT

Item no.	PRS-34-106-100ug
Manufacturer	ProSci
Amount	100 ug
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Format	Liquid
Applications	WB, IHC
Specific against	Human (Homo sapiens)
Host	Mouse
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	ESR1 Antibody: ER, ESR, Era, ESRA, ESTRR, NR3A1, Estrogen receptor, ER-alpha, ER
Available
Shipping	blue ice or RT
By Research Area	Signal Transduction
Immunogen	Recombinant human Estrogen Receptor alpha protein (amino acids 2-185) was used as the immunogen for this Estrogen Receptor alpha antibody.
Applications	Western blot: 0.5-1 ug/ml Immunohistochemistry (FFPE): 1-2 ug/ml for 30 minutes at RT (1) Prediluted format: incubate for 30 min at RT (2) The optimal dilution of the Estrogen Receptor antibody for each application should be determined by the researcher. 1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Purification	Protein G affinity chromatography
Clonality	Monoclonal
Clone	SPM567
Isotype	IgG1, kappa
Conjugate	Unconjugated
Buffer	PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration	0.2 mg/mL
Storage Conditions	Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	ESR1
Ncbi Gene Id #	2099
User Note	Optimal dilutions for each application to be determined by the researcher
Ncbi Official Symbol	ESR1
Ncbi Official Full Name	estrogen receptor 1
Ncbi Organism	homo sapiens
Swissprot #	P03372
Background	This mAb is specific to ER alpha and shows minimal cross-reaction with other members of the family. ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy.
1st Image Caption	Formalin-fixed, paraffin-embedded human breast carcinoma stained with Estrogen Receptor antibody (SPM567).
2nd Image Caption	Western blot of Estrogen Receptor in MCF-7 lysate using Estrogen Receptor antibody (SPM567).

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 100 ug

Listprice: €788.00

Price: €788.00

available

Delivery expected until 10/2/2025

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