Comparison

CD20 Antibody [SPM618]

€788.00
Excl. VAT
Item no. PRS-33-285-100ug
Manufacturer ProSci
Amount 100 ug
Category
Type Antibody Monoclonal
Format Liquid
Applications FC, IF, IHC
Specific against Human (Homo sapiens)
Host Mouse
ECLASS 10.1 42030590
ECLASS 11.0 42030590
UNSPSC 12352203
Alias B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1, CD20, MS4A1, CD20
Available
Shipping
blue ice or RT
By Research Area
Cancer, Immunology, Signal Transduction
Immunogen
Recombinant human MS4A1/CD20 protein was used as the immunogen for this anti-CD20 antibody.
Applications
Flow Cytometry: 0.5-1 ug/million cells

IF: 0.5-1 ug/ml

Immunohistology (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)

Prediluted format : incubate for 30 min at RT (2)

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the anti-CD20 antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Purification
Protein G affinity chromatography
Clonality
Monoclonal
Clone
SPM618
Isotype
IgG2a, kappa
Conjugate
Unconjugated
Buffer
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration
0.2 mg/mL
Storage Conditions
Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications
None
Ncbi Official Symbol
MS4A1
Ncbi Gene Id #
931
User Note
Optimal dilutions for each application to be determined by the researcher
Ncbi Official Symbol
MS4A1
Ncbi Official Full Name
membrane-spanning 4-domains, subfamily A, member 1
Ncbi Organism
Homo sapiens
Swissprot #
P11836
Background
This antibody recognizes a protein of 33-37kDa, identified as CD20. The epitope is similar to or identical to that recognized by other CD20 antibodies including Leu-16 and B1. This antibody can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood, Bcell localization in tissues and B lymphocyte purification by immunosorbent methods. CD20 is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. It is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. Protein passes through the membrane 4 times with both ends in cytoplasm and exposes one short and one longer loop to the external environment. CD20 is not glycosylated in resting B-cells and its cytoplasmic domains are differentially phosphorylated upon activation. It acts as a calcium channel involved in B-cell activation and cell cycle progression.
1st Image Caption
IHC testing of anti-CD20 antibody and FFPE human tonsil tissue

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 100 ug
Available: In stock
Listprice: €788.00
Price: €788.00
available

Delivery expected until 10/2/2025 

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