« Back
Home /
ACE2 Antibody (biotin)

ACE2 Antibody (biotin)

€159.00

Excl. VAT

Item no.	PRS-3217-biotin-0.02mg
Manufacturer	ProSci
Amount	0.02 mg
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Format	Liquid
Applications	ELISA
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	ACE2 Antibody: ACEH, Angiotensin-converting enzyme 2, ACE-related carboxypeptidase, ACEH, SARS-CoV receptor, SARS-CoV-2 receptor
Available
Shipping	blue ice or RT
By Research Area	Infectious Disease
Homology	Predicted species reactivity based on immunogen sequence: Bovine: (88%)
Immunogen	Anti-ACE2 antibody (biotin) (3217) was raised against a peptide corresponding to 18 amino acids near the carboxy terminus of human ACE2. The immunogen is located within the last 50 amino acids of ACE2.
Specificity	Anti-ACE2 has no cross response to ACE1.
Positive Control 1	Cat. No. 1305 - Human Kidney Tissue Lysate
Positive Control 2	Cat. No. 10-401 - Human Kidney Tissue Slide
Positive Control 3	Cat. No. 1306 - Human Spleen Tissue Lysate
Positive Control 4	Cat. No. 1313 - Human Testis Tissue Lysate
Predicted Molecular Weight	Predicted: 93kD Observed: 130 kD (7 N-linked glycosylation)
Isoforms	Human ACE2 has 2 isoforms, including isoform 1 (805aa, 93kD) and isoform 2 (555aa, 64kD). Mouse ACE2 also has 2 isoforms, including isoform 1 (805aa, 92kD) and isoform 2 (353aa, 40kD). Rat ACE2 has one isoform (805aa, 93kD). 3215 can detect human, mouse and rat.
Purification	ACE2 Antibody is affinity chromatography purified via peptide column.
Clonality	Polyclonal
Isotype	IgG
Conjugate	biotin
Buffer	ACE2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration	1 mg/mL
Storage Conditions	ACE2 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Related Products	Cat. No. 3217P – ACE2 Peptide
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	ACE2
Accession #	NP_068576
Protein Gi #	11225609
Ncbi Gene Id #	59272
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	ACE2
Ncbi Official Full Name	angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
Ncbi Organism	Homo sapiens
Swissprot #	Q9BYF1
Background	ACE2 Antibody: Angiotensin-converting enzyme 2 (ACE2) plays a central role in vascular, renal, and myocardial physiology. In contrast to its homolog ACE, ACE2 expression is restricted to heart, kidney, and testis. Recently. ACE2 has also been shown to be a functional receptor of the SARS coronavirus. Homology modeling shows 2019-nCoV has a similar receptor-binding domain structure as SARS-CoV, which suggests COVID-19 (2019-nCoV) may use ACE2 as a receptor in humans for infection. The normal function of ACE2 is to convert the inactive vasoconstrictor angiotensin I (AngI) to Ang1-9 and the active form AngII to Ang1-7, unlike ACE, which converts AngI to AngII. While the role of these vasoactive peptides is not well understood, lack of ACE2 expression in ace2-/ace2- mice leads to severely reduced cardiac contractility, indicating its importance in regulating heart function.
Background References 1	Donoghue et al. Circ. Res. 2000; 87:1-9.
Background References 2	Tipnis et al. J Biol. Chem. 2000; 275:33238-43.
Background References 3	Li et al. Nature 2003; 426:450-4.
Background References 4	Lu et al. The Lancet 2020 (published online).
Background References 5	Crackower et al. Nature 2002; 417:822-8.
Citation 1	Wei, et al.Genome-wide CRISPR Screens Reveal Host Factors Critical for SARS-CoV-2 Infection. Cell. 2020 Oct 20; S0092-8674(20)31392-1. doi: 10.1016/j.cell.2020.10.028.PMID: 33147444
1st Image Caption	Figure 1 ELISA Validation Coating Antigen: immunogen peptide, 3217P, 10 μ g/mL, incubate at 4 ˚ C overnight.Detection Antibodies: SARS-CoV-2 Spike antibody, 3217-biotin or 3217, dilution: 0.5-1000 ng/mL, incubate at RT for 1 hr. 3217-biotin was detected by HRP-conjugated streptavidin at 1:5, 000 and 3217 was detected by anti-rabbit HRP conjugated secondary antibodies at 1:10, 000 , incubate at RT for 1 hr.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

Request product datasheet

Request safety datasheet

Simple Order Order Subscription

Amount: 0.02 mg

Listprice: €159.00

Price: €159.00

available

Delivery expected until 9/4/2025

Quantity:

Compare