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Ki67 Antibody

Ki67 Antibody

€451.00

Excl. VAT

Item no.	PRS-18-495-50uL
Manufacturer	ProSci
Amount	50 uL
Category	Oncology Neuroscience Neural Lineage Markers Metabolism Molecular Targets for Neuroscience Neural Development Antibodies By Cell Type Blastoma Carcinoma Hematology Lymphoma Cell Biology Obesity Cell Status Immunohistochemistry Western Blot Immunofluorescence Proliferation Viability Primary Antibodies By Organ Lung Cancer Bladder Cancer Breast Cancer Testicular & Prostate Cancer Cytoskeleton Markers
Type	Antibody Primary
Format	Liquid
Applications	WB, IF, IHC
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	monoclonal Ki-67, Proliferation-related Ki-67 antigen, Ki-67, KIA, HGNC:7107, antigen identified by monoclonal Ki-67, MKI67, MIB-1
Available
Shipping	blue ice or RT
By Research Area	Cancer, Cell Cycle, Neuroscience
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1200-1300 of human Ki67 (NP_001139438.1).
Applications	WB: 1:500 - 1:2000 IHC: 1:50 - 1:100 IF: 1:50 - 1:100
Positive Control 1	HeLa
Positive Control 2	PC-3
Positive Control 3	NIH/3T3
Predicted Molecular Weight	Observed: 360kDa
Purification	Affinity purification
Clonality	Polyclonal
Isotype	IgG
Conjugate	Unconjugated
Buffer	PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage Conditions	Store at -20˚ C. Avoid freeze / thaw cycles.
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	MKI67
Ncbi Gene Id #	4288
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	MKI67
Ncbi Official Full Name	marker of proliferation Ki-67
Ncbi Organism	Homo sapiens
Swissprot #	P46013
Background	This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X.
1st Image Caption	Western blot analysis of extracts of various cell lines, using Ki67 antibody (18-495) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
2nd Image Caption	Immunohistochemistry of paraffin-embedded human lung cancer using Ki67 antibody (18-495) at dilution of 1:200 (40x lens).
3rd Image Caption	Immunofluorescence analysis of HeLa cells using Ki67 antibody (18-495) at dilution of 1:100. Blue: DAPI for nuclear staining.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 50 uL

Listprice: €451.00

Price: €451.00

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Delivery expected until 9/4/2025

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