SARS-CoV-2 (COVID-19) Nucleocapsid Antibody [7A7]

Infectious Disease, COVID-19

WB(1:500-2000), ELISA(1:5000-1000)

Monoclonal

IgG2b

0.01M PBS(pH7.4) with 0.1% Proclin300

Store at 4˚ C short term. Aliquot and store at -20˚ C long term. Avoid freeze-thaw cycles.

None

Optimal dilutions for each application to be determined by the researcher.

Nucleocapsid phosphoprotein

Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.