Anti-Mouse CD366 (Tim-3) - Purified

Antibody Details

Product Details

Reactivity Species

Mouse

Host Species

Rat

Immunogen

This antibody was produced using recombinant mouse TIM-3

Product Concentration

0.5 mg/ml

Formulation

This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.

Product Preparation

Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

Storage and Handling

This purified antibody is stable when stored at 2-8°C. Do not freeze.

Country of Origin

USA

Shipping

Next Day 2-8°C

RRID

AB_2892978

Applications and Recommended Usage?
Quality Tested by Leinco

FC The suggested concentration for CD366 antibody (clone RMT3-23) for staining cells in flow cytometry is ≤ 1.0 μg per 10⁶ cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application.

Other Applications Reported In Literature ?

B

IHC

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Clone RMT2-23 recognizes an epitope on mouse Tim-3.

Antigen Distribution

Tim-3 is expressed on activated Th1 and Tc1 lymphocytes, CD11b+ macrophages, Tregs, NK cells, and mast cells.

Background

Tim-3 antibody, clone RMT3-23, recognizes T cell immunoglobulin and mucin domain-containing protein 3 (TIM3, also known as CD366), a TIM family member of immunoregulatory proteins. Tim-3 is a 60kDa type I transmembrane protein with an extracellular immunoglobulin and mucin-like domain and a cytoplasmic tyrosine phosphorylation motif. Tim-3 is expressed by IFNg-producing Th1 CD4 and Tc1 (cytotoxic) CD8 T cells¹, regulatory T cells (Tregs)², myeloid cells³, NK cells⁴, and mast cells⁵. Tim-3 is an inhibitory molecule that limits Th1-mediated inflammatory diseases, including a model of central nervous system inflammation (experimental autoimmune encephalomyelitis, EAE)¹, inflammatory bowel disease (IBD)⁶, and type I diabetes⁷. Tim-3 is also proposed to induce immunological tolerance⁷, promote Th1 apoptosis⁸, and regulate macrophage activation1. In addition, Tim-3 is upregulated on tumor-infiltrating lymphocytes, and coblockade of Tim-3 and immune checkpoint inhibitors, such as PD-1, is currently being investigated in clinical trials for the treatment of cancer⁹.

Antigen Details

Protein

Tim-3

Ligand/Receptor

Putative ligand on resting CD4+ lymphocytes

Function

May play a role in the development of immune responses and the development of Th1-mediated responses

PubMed

Tim-3

NCBI Gene Bank ID

171285 /a>

UniProt.org

Information on Uniprot.org

Research Area

Immunology

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Inhibitory Molecules

References & Citations

1. Monney, L. et al. (2002)Nature 415, 536–541.

2. Gao, X. et al. (2012) PLOS ONE 7, e30676.

3. Anderson, A. C. et al. (2007) Science 318, 1141–1143.

4. Ndhlovu, L. C. et al. (2012) Blood 119, 3734–3743.

5. Phong, B. L. et al. J. (2015) Exp. Med. 212, 2289–2304.

6. Li, X. et al. (2010) Clin. Immunol. 134, 169–177.

7. Sanchez-Fueyo, A. et al. (2003) Nat. Immunol. 4, 1093–1101.

8. Zhu C, et al.. Nature Immunology. 2005; 6:1245–1252.

Technical Protocols