Anti-Mouse RANKL (CD254) - Purified In Vivo GOLD Functional Grade

Antibody Details

Product Details

Reactivity Species

Mouse

Host Species

Rat

Immunogen

Purified Recombinant Mouse RANKL (NSO-derived)

Product Concentration

≥ 5.0 mg/ml

Endotoxin Level

≤ 1.0 EU/mg as determined by the LAL method

Purity

≥95% monomer by analytical SEC

⋅

> 95% by SDS Page

Formulation

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.

Product Preparation

Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

Storage and Handling

Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Country of Origin

USA

Shipping

Next Day 2-8°C

RRID

AB_2831709

Applications and Recommended Usage?
Quality Tested by Leinco

FC The suggested concentration for RANKL antibody, clone IK22/5 for staining cells in flow cytometry is ≤ 0.25 μg per 10⁶ cells in a volume of 100 μl or 100 μl of whole blood. Titration of the reagent is recommended for optimal performance for each application.

WB The suggested concentration for this RANKL antibody for use in western blotting is 1-10 μg/ml.

Other Applications Reported In Literature ?

B

IP

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Clone IK22/5 recognizes an epitope on mouse RANKL.

Antigen Distribution

RANKL is expressed on activated T cells and osteoclasts.

Background

RANKL antibody, clone IK22/5, recognizes receptor activator of NF-κB ligand (RANKL), also known as CD254, TNF-related activation-induced cytokine (TRANCE), tumor necrosis factor ligand superfamily member 11 (TNFSF11), osteoprotegerin ligand (OPGL), and osteoclast differentiation factor (ODF). RANKL is a type II transmembrane protein belonging to the TNF cytokine superfamily and binds to the signaling receptor, RANK, or decoy receptor, osteoprotegerin (OPG). RANKL is expressed on osteoclasts and is essential for osteoclast differentiation, activation, and survival¹. RANKL is also expressed on activated T cells and regulates T cell-dendritic cell (DC) interactions, enhancing DC activation and survival^2-3. In addition, RANKL is crucial for lymph node, bone marrow, and thymus development⁴. Treatment with denosumab, an antibody directed against RANKL, is approved for the treatment of patients at high risk for bone fractures, including osteoporosis, and has shown anti-tumor effects^5.

Antigen Details

Protein

RANKL

Ligand/Receptor

RANK, OPG

PubMed

RANKL

NCBI Gene Bank ID

21943

UniProt.org

Information on Uniprot.org

Research Area

Cell Biology

.

Immunology

References & Citations

1. Park JH, et al. (2017) Mol Cells. 40(10):706-713

2. Wong BR, et al. (1997) J Biol Chem. 272(40):25190-4

3. Anderson, D. M., et al. (1997) Nature 390:175.

4. Ono T, et al. (2020) Inflamm Regen. 2020; 40:2.

5. de Groot AF, at al. (2018) Cancer Treat Rev. 62:18-28

Technical Protocols