Donkey F(ab’)2 Anti-Rabbit IgG (H&L) (Adsorbed Against: Ms., Hu., Rt., Bv., Shp., Hs., GP., CK., Sy. Hms.) - Biotin

Antibody Details

Product Details

Reactivity Species

Rabbit

Host Species

Donkey

Immunogen

Purified Rabbit IgG (H& L) (> 98%)

Product Concentration

1.1 mg/ml

Formulation

This secondary antibody is lyophilized from 0.01M phosphate buffered saline (PBS) pH 7.6 containing 0.25M NaCl, 15 mg/ml IgG free BSA and 0.05% sodium azide as a preservative.

Product Preparation

This F(ab')₂ secondary antibody was generated by optimal pepsin degestion of the antisera followed by antigen affinity chromatography to elimiate the presence of Fc fragment and whole molecules.

Storage and Handling

This lyophilized secondary antibody conjugate should be stored desiccated at 2-8°C. The reconstituted Biotin conjugate containing glycerol should be stored at -20°C in a manual defrost freezer.

Expiration Date

One year from date of reconstitution

Country of Origin

USA

Shipping

Next Day 2-8°C

Working Concentration

WB: 1:20, 000 - 1:400, 000ELISA: 1:20, 000 - 1:400, 000IHC: 1:500 - 1:5, 000FC: 1:200 - 1:1, 000

RRID

AB_2894186

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Donkey F(ab')₂ Anti-Rabbit IgG (H& L) recognizes Rabbit IgG heavy and light chains. This secondary antibody was purified using antigen affinity chromatography and adsorbed against serum proteins and immunoglobulins to minimize species cross-reactivity.

Antigen Details

Protein

IgG (H& L)

PubMed

IgG (H& L)