Goat F(ab')2 Anti-Mouse IgG (H&L) (Adsorbed Against: Hu., Bv., Hs.) - APC

Antibody Details

Product Details

Reactivity Species

Mouse

Host Species

Goat

Immunogen

Purified mouse IgG containing all isotypes.

Product Concentration

0.5 mg/ml

Formulation

This secondary antibody is lyophilized from 0.01M phosphate buffered saline (PBS) pH 7.6 containing 0.25M NaCl, 15 mg/ml IgG free BSA and 0.05% sodium azide as a preservative.

Product Preparation

This F(ab')₂ secondary antibody was generated by optimal pepsin degestion of the antisera followed by antigen affinity chromatography to elimiate the presence of Fc fragment and whole molecules.

Storage and Handling

This lyophilized secondary antibody conjugate should be stored desiccated at 2-8°C. The reconstituted Allophycocyanin (APC) conjugate should be stored at 2-8°C. Do Not Freeze.

Expiration Date

One year from date of reconstitution

Country of Origin

USA

Shipping

Next Day Ambient

Working Concentration

It is recommended that working dilutions be prepared fresh daily. A working dilution of 1:50 - 1:200 is recommended for most applications.

Excitation Laser

Red Laser (633 nm)

RRID

AB_2894038

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Goat F(ab')₂ Anti-Mouse IgG (H& L) recognizes Mouse IgG (H& L). This secondary antibody was purified using antigen affinity chromatography and adsorbed against serum proteins and immunoglobulins to minimize species cross-reactivity.

Antigen Details

Protein

IgG (H& L)

PubMed

IgG (H& L)