Anti-Human HLA A2,B7 (MHC Class I) - Purified In vivo GOLD Functional Grade

Antibody Details

Product Details

Reactivity Species

Human

Host Species

Mouse

Immunogen

Solubilized HLA-B7 antigen

Product Concentration

≥ 5.0 mg/ml

Endotoxin Level

≤ 1.0 EU/mg as determined by the LAL method

Purity

≥95% monomer by analytical SEC

⋅

> 95% by SDS Page

Formulation

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.

Product Preparation

Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

Storage and Handling

Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Country of Origin

USA

Shipping

Next Day 2-8°C

RRID

AB_2893725

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Clone BB7.6 recognizes an epitope shared by the human MHC class I molecule HLA-B.

Antigen Distribution

HLA-B is ubiquitously expressed on nucleated cells.

Background

HLA-B antibody, clone BB7.6, recognizes a shared epitope (Bw6) of the major histocompatibility complex (MHC) class I molecule human leukocyte antigen (HLA)-B¹. MHC class I is ubiquitously expressed on the cell surface of nucleated cells and consists of a 45-kDa type I transmembrane glycoprotein (α-chain or heavy chain) and a 12-kDa soluble protein (β2-microglobulin, β2M)^{2, 3}. The α-chain consists of three domains (α1, α2, and α3)⁴. α1 and α2 form the closed antigen-binding groove and bind to 8-10 aa peptides derived from cytosolic antigens^5-7. β2M noncovalently associates with α3, which is essential for MHC stability. MHC class I plays a critical role in the adaptive immune response by presenting endogenous antigens to cytotoxic CD8 T cells. MHC class I molecules can also present exogenous antigens to CD8 T cells via a process known as cross-presentation⁸. The T cell receptor (TCR)/CD3 complex of CD8 T cells interacts with peptide-MHC class I, which induces CD8 T cell activation and subsequent cell-killing. CD8 molecules also bind to MHC class I, which helps augment TCR signaling⁹. In contrast to CD8 T cells, MHC class I is an inhibitory ligand for natural killer (NK) cells, promoting self tolerance¹⁰. MHC class I also contributes to the positive selection of CD8 T cells and NK cell specificity^{11, 12}.

Antigen Details

Protein

HLA-A2, B7

PubMed

HLA-A2, B7

Research Area

Immunology

References & Citations

1. Lutz CT, et al. (1994) J Immunol. 153(9):4099-110.

2. Mitaksov V & Fremont DH. (2006) J Biol Chem. 281(15):10618-25.

3. Wieczorek M, et al. (2017) Front Immunol. 8:292.

4. Jones EY. (1997) Curr Opin Immunol. 9(1):75-9.

5. Matsumura M, et al (1992) Science. 257:927–34.10.1126/science.1323878

6. Bouvier M & Wiley DC. (1994) Science. 398–402.10.1126/science.8023162

7. Zacharias M & Springer S. (2004) Biophys J. 87:2203–14.10.1529/biophysj.104.044743

8. Cruz FM, et al. (2017) Annu Rev Immunol. 35:149-176.

9. Artyomov MN, et al. (2010) Proc Natl Acad Sci USA. 107(39):16916-16921.

10. Orr MT & Lanier LL. (2010) Cell. 142(6):847-856.

11. Raulet DH. (1994) Adv Immunol. 55:381-421.

12. Salcedo M & Ljunggren HG. (1996) Chem Immunol. 64:44-58.

Technical Protocols