Anti-Mouse H-2Kd (MHC Class I) - Purified In vivo GOLD Functional Grade

Antibody Details

Product Details

Reactivity Species

Mouse

Host Species

Mouse

Product Concentration

≥ 5.0 mg/ml

Endotoxin Level

≤ 1.0 EU/mg as determined by the LAL method

Purity

≥95% monomer by analytical SEC

⋅

> 95% by SDS Page

Formulation

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.

Product Preparation

Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

Storage and Handling

Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Country of Origin

USA

RRID

AB_2737514

Applications and Recommended Usage?
Quality Tested by Leinco

FC The suggested concentration for clone A4C8.1-Do9 antibody for staining cells in flow cytometry is ≤ .25 μg per 10⁶ cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application.

Other Applications Reported In Literature ?

WB

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Clone A4C8.1-Do9 recognizes an epitope on mouse MHC class I H-2K haplotype d.

Antigen Distribution

H-2Kd is ubiquitously expressed on nucleated cells from mice of the H-2Kd haplotype, including BALB/c.

Background

H-2Kd antibody, clone A4C8.1-Do9, recognizes the major histocompatibility complex (MHC) class I H-2K haplotype d (H-2Kd). MHC class I is ubiquitously expressed on the cell surface of nucleated cells and consists of a 45-kDa type I transmembrane glycoprotein (α-chain or heavy chain) and a 12-kDa soluble protein (β2-microglobulin, β2M)^{1, 2}. The α-chain consists of three domains (α1, α2, and α3)³. α1 and α2 form the closed antigen-binding groove and bind to 8-10 aa peptides derived from cytosolic antigens^4-6. β2M noncovalently associates with α3, which is essential for MHC stability. H-2Kd plays a critical role in the adaptive immune response by presenting endogenous antigens to cytotoxic CD8 T cells. MHC class I molecules can also present exogenous antigens to CD8 T cells via a process known as cross-presentation⁷. The T cell receptor (TCR)/CD3 complex of CD8 T cells interacts with peptide-MHC class I, which induces CD8 T cell activation and subsequent cell-killing. CD8 molecules also bind to MHC class I, which helps augment TCR signaling⁸. In contrast to CD8 T cells, MHC class I is an inhibitory ligand for natural killer (NK) cells, promoting self tolerance⁹. MHC class I also contributes to the positive selection of CD8 T cells and NK cell specificity^{10, 11}.

Antigen Details

Protein

MHC Class I

PubMed

MHC Class I

Research Area

Immunology

.

Innate Immunity

References & Citations

1. Mitaksov V & Fremont DH. S (2006) J Biol Chem. 281(15):10618-25

2. Wieczorek M, et al. (2017) Front Immunol. 8:292.

3. Jones EY. (1997) Curr Opin Immunol. 9(1):75-9

4. Matsumura M, et al. (1992) Science. 927–34.10.1126/science.1323878

5. Bouvier M & Wiley DC. (1994) Science. 265:398–402.10.1126/science.8023162

6. Zacharias M & Springer S. (2004) Biophys J. 87:2203–14.10.1529/biophysj.104.044743

7. Cruz FM, et al. (2017) Annu Rev Immunol. 35:149-176

8. Artyomov MN, et al. (2010) Proc Natl Acad Sci USA. 07(39):16916-16921

9. Orr MT & Lanier LL. (2010) Cell. 142(6):847-856

10. Raulet DH. (1994) Adv Immunol. 55:381-421.

11. Salcedo M & Ljunggren HG. (1996) Chem Immunol. 1996; 64:44-58

Technical Protocols