Donkey F(ab’)2 Anti-Goat IgG (H&L)-R-PE

Antibody Details

Product Details

Reactivity Species

Goat

Host Species

Donkey

Immunogen

Purified Goat IgG (H& L) (> 98%)

Product Concentration

0.5 mg/ml

Formulation

This secondary antibody is lyophilized from 0.01M phosphate buffered saline (PBS) pH 7.6 containing 0.25M NaCl, 15 mg/ml IgG free BSA and 0.05% sodium azide as a preservative.

Product Preparation

This F(ab')₂ secondary antibody was generated by optimal pepsin degestion of the antisera followed by antigen affinity chromatography to elimiate the presence of Fc fragment and whole molecules.

Storage and Handling

This lyophilized secondary antibody conjugate should be stored desiccated at 2-8°C. The reconstituted R-phycoerythrin (R-PE) conjugate should be stored at 2-8°C. Do Not Freeze.

Expiration Date

One year from date of reconstitution

Country of Origin

USA

Shipping

Next Day Ambient

Working Concentration

It is recommended that working dilutions be prepared fresh daily. FC: 1:50 - 1:200

Excitation Laser

Blue Laser (488 nm) and/or Green Laser (532 nm)/Yellow-Green Laser (561 nm)

RRID

AB_2893615

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Donkey F(ab')₂ Anti-Goat IgG (H& L) recognizes Goat IgG (H& L). This secondary antibody was purified using antigen affinity chromatography and has been adsorbed against serum proteins and immunoglobulins to minimize species cross-reactivity.

Antigen Details

Protein

IgG (H& L)

PubMed

IgG (H& L)