Anti-Human Alpha Defensin-5 (HD5) - Purified In vivo PLATINUM Functional Grade

Antibody Details

Product Details

Reactivity Species

Human

Host Species

Mouse

Immunogen

Recombinant Human HD5 (aa 20-94).

Endotoxin Level

< 0.5 EU/mg as determined by the LAL method

Purity

≥98% monomer by analytical SEC

⋅

> 95% by SDS Page

Formulation

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.

Product Preparation

Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

Pathogen Testing

To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.

Storage and Handling

Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Country of Origin

USA

Shipping

Next Day 2-8°C

RRID

AB_2829964

Applications and Recommended Usage?
Quality Tested by Leinco

WB
ELISA

Other Applications Reported In Literature ?

IHC FFPE
Dot

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Clone 8C8 recognizes human alpha-defensin 5 (HD5).

Antigen Distribution

HD5 is highly expressed in the secretory granules of Paneth cells of the ileum.

Background

The colon lies adjacent to the small intestine and has heavy bacterial colonization from ingested food and water that is often contaminated with bacteria. Remarkably, the small intestine has a low microbial density. A special type of epithelial cell, called a Paneth cell, can be found clustered at the base of the tubular glands that lie between the villi of the inner surface of the small intestine. These cells secrete defensins which have been shown to have activity against both Gram-positive and Gram-negative microbes.³ Six human α-defensins, a subfamily of defensin peptides characterized by their cysteine spacing and disulfide connectivity², have been identified. Human Paneth cells express α-defensin 5 (HD5) along with HD6. Paneth cells are most numerous in the ileum and have many features similar to those of myeloid cells. They are multifaceted cells with a large quantity of apically-located eosinophilic secretory granules containing lysozyme and other antimicrobial factors which are released upon bacterial stimulation.² In addition, these cells express tumor necrosis factor alpha (TNF-a), CD1, and CD15.¹ Paneth cells do not store defensins as fully processed or active peptides, rather they store them as inactive propeptides.² They are released as mature peptides after trypsin digestion. Trypsin is also secreted from the Paneth cell granules. It has been reported that disrupted α-defensin processing in murine Paneth cells has initiated a vulnerability to enteric infection.² HD5 expression levels have been found to be negatively correlated to intestinal infection. Additionally, studies have shown HD5 to be a strong antagonist towards human Papillomavirus infection. Furthermore, low expression of HD5 is thought to play a role in Crohn’s disease.³ Anti-Human Alpha Defensin-5 (HD5) Clone 8C8 has been shown to recognize the propeptide and the partially processed forms of HD5. However, clone 8C8 has been reported to scarcely recognize the mature peptide. There was no reported cross reactivity with HD6, lysozyme, or sPLA2.

Antigen Details

Protein

HD5

PubMed

HD5

NCBI Gene Bank ID

1670

UniProt.org

Information on Uniprot.org

References & Citations

1. Porter, EM. et al. (1997) Infect Immun. 65(6):2389-95.

2. Ghosh, D. et al. (2002) Nat Immunol. 3(6):583-90.

3. Wehkamp, J. et al. (2019) Proc Natl Acad Sci U S A. 116(9): 3746–3751.

4. Schwaderer, AL. et al. (2012) PLoS One. 7(2):e31712.

5. Hains, D. et al. (2016) US Patent Application Publication No. US 2016/0069905 A1

6. Shen, B. et al. (2005) J Clin Pathol. 58(7):687-94.
7. Ranki, A. et al. (2015) Clin Immunol. 158(2):212-20.

8. Hulscher, JB. et al. (2016) Pediatr Res. 80(2):306-10.

Technical Protocols