Anti-Mouse CD1d (Clone D5) - Purified In vivo GOLD Functional Grade

Antibody Details

Product Details

Reactivity Species

Human

Host Species

Mouse

Immunogen

CD1d a1-a2 domains

Product Concentration

≥ 5.0 mg/ml

Endotoxin Level

≤ 1.0 EU/mg as determined by the LAL method

Purity

≥95% monomer by analytical SEC

⋅

> 95% by SDS Page

Formulation

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.

Product Preparation

Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

Storage and Handling

Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Country of Origin

USA

Shipping

Next Day 2-8°C

RRID

AB_2893555

Applications and Recommended Usage?
Quality Tested by Leinco

WB The suggested concentration for this CD1d (clone D5) antibody for use in western blotting is 1-10 μg/ml.

Other Applications Reported In Literature ?

IP

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Clone D5 recognizes an epitope on human CD1d.

Antigen Distribution

CD1d is expressed on monocytes, DCs, macrophages, and epithelial cells.

Background

CD1d antibody, D5, recognizes human CD1d, also known as CD1.1 and Ly-38, a 48 kDa type 1 membrane glycoprotein that belongs to the cluster of differentiation 1 (CD1) family of glycoproteins. CD1d is expressed on epithelial cells and monocyte-derived antigen presenting cells (APCs), including monocytes, DCs, and macrophages^{1, 2}. CD1d is an MHC class I-like molecule involved in the presentation of lipid antigens, such as α-galactosylceramide (α-GalCer), to a highly conserved T cell subset, named invariant natural killer T (iNKT) cells or CD1d-restricted T cells¹. Following activation, iNKT cells rapidly proliferate and produce large amounts of cytokines and cytolytic mediators^{3, 4}.

Antigen Details

Protein

CD1d

PubMed

CD1d

NCBI Gene Bank ID

912

Research Area

Apoptosis

.

Immunology

References & Citations

1. Van Kaer L., et al. (2004) Nat Rev Immunol. 4(3):231-7

2. Porcelli SA., et al. (2000) Eur J Immunol. 30(12):3468-77

3. Teyton L., et al. (2006) Annu Rev Immunol. 2006; 25:297–336

4. Kronenberg M. (2004) Annu Rev Immunol. 23:877–900.

Technical Protocols