Anti-Mouse CD3epsilon (Clone 145-2C11) - DyLight 650

Antibody Details

Product Details

Reactivity Species

Mouse

Host Species

Armenian Hamster

Immunogen

H-2Kb-specific mouse cytotoxic T lymphocyte clone BM10-37

Product Concentration

0.2 mg/ml

Formulation

This DyLight® 650 conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.

Storage and Handling

This DyLight^® 650 conjugate is stable when stored at 2-8°C. Do not freeze.

Country of Origin

USA

Shipping

Next Day 2-8°C

Excitation Laser

Red Laser (652 nm)

RRID

AB_2829328

Applications and Recommended Usage?
Quality Tested by Leinco

FC The suggested concentration for this 145-2C11 antibody for staining cells in flow cytometry is ≤ 0.5 μg per 10⁶ cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.

Additional Reported Applications For Relevant Conjugates ?

IHC (Frozen)

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Clone 145-2C11 recognizes an epitope on mouse CD3ε.

Antigen Distribution

CD3ε is primarily expressed on mature T cells and NK-T cells and at different levels on differentiation-dependent thymocytes.

Background

CD3ε is a 20kDa subunit of the TCR complex and is a transmembrane T-cell surface glycoprotein that belongs to the Ig superfamily. It is one of five polypeptide chains that form the TCR complex by associating with the CD3δ, γ and ζ chains, in addition to the TCR α/β or γ/δ chains. CD3 is involved in TCR signaling, enumeration of immunocompetent T-lymphocytes in peripheral blood, and signal transduction during antigen recognition.

Antigen Details

Protein

CD3ε

Ligand/Receptor

Peptide antigen/MHC-complex

PubMed

CD3ε

NCBI Gene Bank ID

12501

UniProt.org

Information on Uniprot.org

Research Area

Immunology

Technical Protocols