Anti-Mouse CD86 - DyLight 488

Antibody Details

Product Details

Reactivity Species

Mouse

Host Species

Rat

Immunogen

Purified Recombinant Mouse CD86 (> 98%)

Product Concentration

0.2 mg/ml

Formulation

This DyLight® 488 conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.

Storage and Handling

This DyLight^® 488 conjugate is stable when stored at 2-8°C. Do not freeze.

Country of Origin

USA

Excitation Laser

Blue Laser (493 nm)

RRID

AB_2829248

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Clone GL-1 recognizes an epitope on mouse CD86.

Antigen Distribution

CD86 is expressed on activated B and T cells, macrophages, dendritic cells, and astrocytes.

Background

CD86 is an 80kD Ig superfamily member that is involved in immunoglobulin class-switching and activation of NK cell-mediated cytotoxicity. CD80 is closely related to, and works in tandem with CD86 to prime T- cells. CD86 is expressed earlier in the immune response than CD80. The ligation of CD28 on T cells with CD80 and CD86 on APCs co-stimulates T cells resulting in enhanced cell activation, proliferation, and cytokine production. CD86 can also bind to CTLA-4 to deliver an inhibitory signal to T cells.

Antigen Details

Protein

B7-2

Ligand/Receptor

CD28, CD152 (CTLA-4)

PubMed

B7-2

NCBI Gene Bank ID

12524

UniProt.org

Information on Uniprot.org

Research Area

Cell Biology

.

Costimulatory Molecules

.

Immunology

.

Neuroinflammation

.

Neuroscience Cell Markers

References & Citations

1. Hathcock, K.S. et al.. (1993) Science 262(5135:905-7)

Technical Protocols