Anti-Human CD3 - DyLight 488

Antibody Details

Product Details

Reactivity Species

Human

Host Species

Mouse

Immunogen

Purified Recombinant Human CD3 (> 98%)

Product Concentration

0.2 mg/ml

Formulation

This DyLight® 488 conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.

Storage and Handling

This DyLight^® 488 conjugate is stable when stored at 2-8°C. Do not freeze.

Country of Origin

USA

Shipping

Next Day 2-8°C

Excitation Laser

Blue Laser (493 nm)

RRID

AB_2829104

Applications and Recommended Usage?
Quality Tested by Leinco

FC The suggested concentration for this UCHT-1 antibody for staining cells in flow cytometry is ≤ .25 μg per 10⁶ cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application.

Other Applications Reported In Literature ?

IF

IHC (Frozen) The suggested concentration for this UCHT-1 antibody in IHC staining on frozen tissue is 5.0 - 10 μg per ml. Titration of the reagent is recommended for optimal performance for each application.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Clone UCHT-1 recognizes an epitope on human CD3ε.

Antigen Distribution

CD3 is expressed on human peripheral blood lymphocytes, splenic lymphocytes, and the majority of T-CLL and T-ALL cells.

Background

CD3ε is a 20kDa subunit of the TCR complex and is a transmembrane T-cell surface glycoprotein that belongs to the Ig superfamily. It is one of five polypeptide chains that form the TCR complex by associating with the CD3δ, γ and ζ chains, in addition to the TCR α/β or γ/δ chains. CD3 is involved in TCR signaling, enumeration of immunocompetent T-lymphocytes in peripheral blood, and signal transduction during antigen recognition.

Antigen Details

Protein

CD3

Ligand/Receptor

Peptide antigen bound to MHC

PubMed

CD3

NCBI Gene Bank ID

915

UniProt.org

Information on Uniprot.org

Research Area

Immunology

References & Citations

1. van Dongen, J. et al. (1988) Blood 71:603
2. Meuer, SC. et al. (1983) Nature 303:808
3. Beverly, P. et al. (1981) Eur. J. Immunol. 11:329
4. Leukocyte Typing III: Code No. 126, 208 and 471; 4th Workshop: Paper No. T3.2

Technical Protocols