Anti-Human CD10 - DyLight 650

Antibody Details

Product Details

Reactivity Species

Human

Host Species

Mouse

Immunogen

Purified Recombinant Human CD10 (> 98%)

Product Concentration

0.2 mg/ml

Formulation

This DyLight® 650 conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.

Storage and Handling

This DyLight^® 650 conjugate is stable when stored at 2-8°C. Do not freeze.

Country of Origin

USA

Shipping

Next Day 2-8°C

Excitation Laser

Red Laser (652 nm)

RRID

AB_2829069

Applications and Recommended Usage?
Quality Tested by Leinco

FC The suggested concentration for this SJ5-1B4 antibody for staining cells in flow cytometry is ≤ 1.0 μg per 10⁶ cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Mouse Anti-Human CD10 (Clone SJ5-1B4) recognizes an epitope on Human CD10. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Antigen Distribution

The CD10 antigen is found on cells from subjects with acute B-lymphoid leukemia.³ The antigen is also expressed in various degrees on progenitor B-cells, renal epithelium, fibroblasts, granulocytes, early T-lymphocytes and germinal center cells.

Background

Anti-Human CD10 may be used in studies characterizing non-T-cell common acute lymphoblastic leukemias or differentiation of early lymphocyte lineages.

Antigen Details

Protein

CD10

Ligand/Receptor

Biologically active peptides including opioid enkephalins, bradykinin, angiotensions I & II

PubMed

CD10

NCBI Gene Bank ID

4311

UniProt.org

Information on Uniprot.org

Research Area

Immunology

References & Citations

1. Leukocyte Typing V (1995) Oxford University Press
2. Billings, R. et al. (1978) J. Natl. Cancer Inst. 61:583
3. Ritz, J. et al. (1980) Nature 283:583
4. Foon, K. A. et al. (1982) Blood 60:1

Technical Protocols