Anti-Mouse CD3epsilon (Clone: 500A2) - DyLight 488

Antibody Details

Product Details

Reactivity Species

Mouse

Host Species

Syrian Hamster

Immunogen

Mouse T cell receptor

Product Concentration

0.2 mg/ml

Formulation

This DyLight® 488 conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.

Storage and Handling

This DyLight^® 488 conjugate is stable when stored at 2-8°C. Do not freeze.

Shipping

Next Day 2-8°C

Excitation Laser

Blue Laser (493 nm)

RRID

AB_2828792

Applications and Recommended Usage?
Quality Tested by Leinco

FC The suggested concentration for this 500A2 antibody for staining cells in flow cytometry is ≤ 0.25 µg per million cells in 100 µl volume. Titration of the reagent is recommended for optimal performance for each application.

Other Applications Reported In Literature ?

IHC (Frozen) The suggested concentration for this 500A2 antibody in IHC staining on frozen tissue is 5.0 - 10 μg per ml. Titration of the reagent is recommended for optimal performance for each application.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Clone 500A2 recognizes an epitope on mouse CD3ε.

Antigen Distribution

CD3ε is primarily expressed on mature T cells and NK-T cells and at different levels on differentiation-dependent thymocytes.

Background

CD3ε is a 20kDa subunit of the TCR complex and is a transmembrane T-cell surface glycoprotein that belongs to the Ig superfamily. It is one of five polypeptide chains that form the TCR complex by associating with the CD3δ, γ and ζ chains, in addition to the TCR α/β or γ/δ chains. CD3 is involved in TCR signaling, enumeration of immunocompetent T-lymphocytes in peripheral blood, and signal transduction during antigen recognition.

Antigen Details

Protein

CD3ε

Ligand/Receptor

Peptide antigen/MHC-complex.

PubMed

CD3ε

NCBI Gene Bank ID

12501

UniProt.org

Information on Uniprot.org

Research Area

Cell Biology

.

Immunology

.

Signal Transduction

References & Citations

1. Havran, WM. et al. (1987) Nature 330:170
2. Allison, JW. et al. (1988) The T-Cell Receptor 33

Technical Protocols