Anti-Mouse CD28 - Purified In vivo PLATINUM Functional Grade

Antibody Details

Product Details

Reactivity Species

Mouse

Host Species

Syrian Hamster

Product Concentration

≥ 5.0 mg/ml

Endotoxin Level

< 0.5 EU/mg as determined by the LAL method

Purity

≥98% monomer by analytical SEC

⋅

> 95% by SDS Page

Formulation

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.

Product Preparation

Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

Pathogen Testing

To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.

Storage and Handling

Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Shipping

Next Day Ambient

RRID

AB_2828714

Applications and Recommended Usage?
Quality Tested by Leinco

FC The suggested concentration for this 37.51 antibody for staining cells in flow cytometry is ≤ 1.0 μg per 10⁶ cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
WB The suggested concentration for this 37.51 antibody for use in western blotting is 1-10 μg/ml.

Other Applications Reported In Literature ?

CyTOF®
IHC (Frozen)
B
Costim
IP

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Clone 37.51 recognizes an epitope on mouse CD28.

Antigen Distribution

CD28 is expressed on thymocytes, CD4+, CD8+ peripheral T cells, NK cells.

Background

CD28 is a 44 kD glycoprotein and a member of the Ig superfamily. In its capacity as a costimulatory receptor, CD28 produces co-stimulatory signals necessary for T cell activation and survival. CD28 is the only B7 receptor constitutively expressed on naive T cells. Without having this CD28/B7 interaction, T cells will fail to respond to their specific antigen when coming in contact with an MHC/antigen complex and thus, are said to be anergic.

Antigen Details

Protein

CD28

Ligand/Receptor

CD80 (B7-1), CD86 (B7-2)

PubMed

CD28

NCBI Gene Bank ID

12487

UniProt.org

Information on Uniprot.org

Research Area

Costimulatory Molecules

.

Immunology

References & Citations

1. Gallimore, A. et al. (2005) J Immunol. 175(11):7098-102. PubMed

2. Gross, J. A. et al. (1992) J. Immunol. 149:380

Technical Protocols