Anti-Human CD86- PE

Antibody Details

Product Details

Reactivity Species

Human

Host Species

Mouse

Formulation

This monoclonal antibody is conjugated to R-phycoerythrin (R-PE) and formulated in modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 0.1% sodium azide as a preservative.

Storage and Handling

This R-phycoerythrin (R-PE) conjugate is stable for at least twelve months when stored at 2-8°C protected from light. Do not freeze.

Country of Origin

USA

Shipping

Next Day 2-8°C

Excitation Laser

Blue Laser (488 nm) and/or Green Laser (532 nm)/Yellow-Green Laser (561 nm)

RRID

AB_2828606

Applications and Recommended Usage?
Quality Tested by Leinco

Flow Cytometry: This conjugate has been pre-diluted to use approximately 10 µl per test when staining cells for flow cytometry. A typical test sample constitutes approximately 50 µl of packed whole blood or 1 x 10⁶ continuous passage or activated cell cultures that have been centrifuged at 500 X g for five minutes and resuspended in 200-400 µl of 1X PBS.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Clone 37301 recognizes an epitope on human CD86.

Antigen Distribution

CD86 is expressed on activated B and T cells, macrophages, dendritic cells, and astrocytes.

Background

CD86 is an 80kD Ig superfamily member that is involved in immunoglobulin class-switching and activation of NK cell-mediated cytotoxicity. CD80 is closely related to, and works in tandem with CD86 to prime T- cells. CD86 is expressed earlier in the immune response than CD80. The ligation of CD28 on T cells with CD80 and CD86 on APCs co-stimulates T cells resulting in enhanced cell activation, proliferation, and cytokine production. CD86 can also bind to CTLA-4 to deliver an inhibitory signal to T cells.

Antigen Details

Protein

CD86

Ligand/Receptor

CD28, CD152

PubMed

CD86

NCBI Gene Bank ID

942

UniProt.org

Information on Uniprot.org

Technical Protocols