Anti-Mouse CD25 - Purified In vivo GOLD Functional Grade

Antibody Details

Product Details

Reactivity Species

Mouse

Host Species

Rat

Immunogen

IL-2-dependent cytolytic mouse T-cell clone B6.1

Product Concentration

≥ 5.0 mg/ml

Endotoxin Level

≤ 1.0 EU/mg as determined by the LAL method

Purity

≥95% monomer by analytical SEC

⋅

> 95% by SDS Page

Formulation

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.

Product Preparation

Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

Storage and Handling

Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Country of Origin

USA

Shipping

Next Day Ambient

RRID

AB_2737451

Applications and Recommended Usage?
Quality Tested by Leinco

FC The suggested concentration for this PC61 antibody for staining cells in flow cytometry is ≤ 1 μg per 10⁶ cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
WB The suggested concentration for this PC61 antibody for use in western blotting is 1-10 μg/ml.

Other Applications Reported In Literature ?

CODEX®
B
Depletion
IHC (Frozen)
IP
FA

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Clone PC61 recognizes an epitope on mouse CD25.

Antigen Distribution

CD25 is expressed in activated T cells and B cells, thymocyte subset, pre-B cells, T regulatory cells.

Background

CD25, a 55 kD type I transmembrane glycoprotein, has been shown to play roles in lymphocyte differentiation, activation, and proliferation. Many resting memory T cells constitutively express IL2RA. It functions as the receptor for HTLV-1, resulting in its expression on neoplastic cells in adult T cell lymphoma/leukemia. CD25 (sIL-2R) has been used to track disease progression. Some additional clinical applications include Chagas disease, a disease characterized by a decline of CD25 expression on immune cells, and Multiple sclerosis, in which treatments with mAbs target CD25.

Antigen Details

Protein

CD25

Ligand/Receptor

IL-2

Function

Forms high affinity IL-2R with IL-2Rβ (CD122) and IL-2Rγ (CD132)

PubMed

CD25

NCBI Gene Bank ID

16184

UniProt.org

Information on Uniprot.org

Research Area

Immunology

References & Citations

1.) Braley-Mullen, H. et al. (2018) Immunohorizons. 2(1): 54–66. PubMed

2.) Leonard, WJ. et al. (2002) The EMBO Journal 21: 3051

3.) Alt, FW. et al. (1995) Immnnity 3: 521

4.) Greene, WC. et al. (1990) J Invest Dermatol. 94: 27S

5.) Gubin et al. (2018) Cell. 175:1014–1030 Journal Link

Technical Protocols