Phospho-H2AFX (S139) Antibody

Histone H2AX (H2a/x) (Histone H2A.X), H2AFX, H2AX

P16104

ELISA, WB, IF, ChIP, Recommended dilution: WB:1:50-1:500, IF:1:1-1:10

WB:1:50-1:500

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Antigen Affinity Purified

Epigenetics and Nuclear Signaling