Anti-Inter alpha trypsin inhibitor

The reactivity of the antiserum is restricted to inter alpha trypsin inhibitor. In immunoelectro- phoresis and radial immunodiffusion, using various antiserum concentrations against human plasma a single precipitin line is obtained and shows a reaction of full identity with the purified immunogen. In precipitating techniques as immunoelectrophoresis and single and double radial immuno- diffusion (Mancini, Ouchterlony) to identify the presence of inter alpha trypsin inhibitor or to determine its concentration. The presence of non-precipitating antibodies has not been assayed. This does not exclude the use of the antiserum in non-precipitating antibody-binding techniques if proper controls are included.

Precipitating polyclonal Sheep anti-Serum to Human inter alpha trypsin inhibitor.

Inter alpha trypsin inhibitor is a proteinase inhibitor with a molecular weight of 160, 000. Its mean concentration in human plasma is about 400g/ml. It has been isolated as a homogenous protein for use in the antiserum production. Freund's complete adjuvant is used in the first step of the immunization procedure.

For Research Use Only.