Vimentin Antibody

Recombinant Protein

This gene encodes a member of the intermediate filament
family. Intermediate filamentents, along with microtubules and
actin microfilaments, make up the cytoskeleton. The protein encoded
by this gene is responsible for maintaining cell shape, integrity
of the cytoplasm, and stabilizing cytoskeletal interactions. It is
also involved in the immune response, and controls the transport of
low-density lipoprotein (LDL)-derived cholesterol from a lysosome
to the site of esterification. It functions as an organizer of a
number of critical proteins involved in attachment, migration, and
cell signaling. Mutations in this gene causes a dominant,
pulverulent cataract.

Polyclonal

7431

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Confocal immunofluorescent analysis of Vimentin Antibody with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

1:50

IF

Confocal immunofluorescent analysis of Vimentin Antibody with U251 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

1:50

WB

All lanes : Anti-VIME Antibody at 1:2000 dilution
Lane 1: Hela whole cell lysate
Lane 2: 293 whole cell lysate
Lane 3: U-2OS whole cell lysate
Lane 4: Jurkat whole cell lysate
Lane 5: K562 whole cell lysate
Lane 6: NIH/3T3 whole cell lysate
Lane 7: C2C12 whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 54 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

1:2000

IHC-P-Leica

Immunohistochemical analysis of paraffin-embedded Human small intestine tissue using AX10005 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

1:500

Rabbit Ig

HUMAN

NP_003371.2