Anti-Human IL-1 Beta Secondary Antibody

Polyclonal

H

80 mg/mL by Refractometry

ELISA 1:500-1:2, 000Neutralization:1:100; Western Blot 1:1, 000; Immunochemistry 1:100-1:200ImmunoPrecipitation:1:400-1:800

Human

Antiserum

None

This antiserum was heated to 56° C for 30 minutes. The antiserum is primarily directed against mature, 17, 000 MW human IL-1ß and is useful in determining its presence in various assays. In general, this antibody also detects primate IL-1ß in the same formats using similar dilutions. The antiserum does not recognize human IL-1a. In ELISA formats and other immunoreactive assays, this antibody will recognize 10% of the non-denatured (native) precursor 31, 000 MW IL-1ß containing samples but will primarily detect all of the 17, 000 MW mature molecule. However, in immunoblot analysis of natural cell products or human body fluids, the usual procedure of hearing the sample in SDS with or without reducing agents will facilitate denaturing of the 31, 000 MW IL- 1ß precursor molecule. Denatured 31, 000 precursor IL-1ß will be recognized by this antibody but often migrates as a 35, 000 MW band. This is due to the unfolding of the denatured precursor IL-1ß exposing epitopes not exposed in the natural state. In immunoblots, depending on the number of cells, the antibody detects the 17, 000 MW band in supernatants as well as a 35, 000 MW band representing the 31, 000 MW IL-1ß precursor in lysates.