Vimentin Antibody (C-term)

Synthetic Peptide

Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.

Polyclonal

7431

Purified Rabbit Polyclonal Antibody (Pab)

Fluorescent confocal image of SY5Y cells stained with Vimentin (C-term) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP2739b Vimentin (C-term) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). Note the highly specific localization of the Vimentin immunosignal to the cytoskeleton, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000026025).

1:100

WB

Western blot analysis of Vimentin antibody (C-term) (Cat.#AP2739b) in NCI-H460 cell line lysates (35ug/lane). Vimentin (arrow) was detected using the purified Pab .

1:1000

IHC-P

Vimentin Antibody (C-term) (Cat. #AP2739b) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

1:10~50

X, B, C, Ha, Pr, M, Pig, Rat

53652

HUMAN

NP_003371.2