Mouse Monoclonal Antibody to Vimentin

Catalog Number: sAP-0092
Product Name: Mouse Monoclonal Antibody to Vimentin
Target Molecle: Vimentin
Aliases: FLJ36605; VIM
Species: Human
MW: N/A
Enterez GeneID: 7431
General Information: Vimentin is the major subunit protein of the intermediate filaments of mesenchymal cells. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis. Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural, muscle and fibroblast origin) compared to carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin antibodies are thus of value in the differential diagnosis of undifferentiated neoplasms and malignant tumors. They are generally used with a panel of other antibodies including those recognising cytokeratins, lymphoid markers, S100, desmin and neurofilaments.
Immunogen: Purified recombinant fragment of Vimentin expressed in E. Coli.
Clone: MM9E7E7;
Isotype: Mouse IgG1
Size and Concentration: 100ug/1mg/ml
Supplied As: Lyophilized Powder from 100ul of Ascitic fluid containing 0.03% sodium azide.
Reconstitution and Storage: Reconstitued with 100ul sterile DI H2O, at stored at 4C or -20C for short or long term storage.
Applications: ELISA: 1 to 10000; WB: 1 to 500 - 1 to 2000; IHC: 1 to 200 - 1 to 1000
References: 1. Xu, N. & Dahlback, B.1999. J. Biol. Chem. 274 (44):31286-31290. ; 2. Christian Wolfrum, Matthew N Poy & Markus Stoffel. 2005. Nat Med. 11(4):418-22. ; 3. Xu, N., Nilsson-Ehle, P. & Ahren, B. 2004. J. Nutr. Biochem. 15 (10):579-582. ; 4. Zhang, X.Y. , et al.2004. Acta Histochem. 106 (2):123-128. ;