Goat anti-LAG3 / CD223 Antibody

Goat anti-LAG3 / CD223 Antibody
Principle Name: LAG3 / CD223
Official Symbol: LAG3
All Name and Symbols: LAG3; lymphocyte activating 3; CD223; lymphocyte-activation gene 3
Accession Number(s): NP_002277.4
Peptide Sequence: EDVSQAQAGTYTCH, is from internal region
Human Gene ID(s): 3902
Non-Human GeneID(s):
Important Comments: This antibody is expected to recognize the extra-cellular domain.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB
Tested Species: Human
Peptide ELISA: antibody detection limit dilution 1 to 2000.
Western Blot: Approx 55kDa band observed in Human Lymph Node and Tonsil lysates (calculated MW of 57.4kDa according to NP_002277.4). Recommended concentration: 0.3-1ug/ml.
Reference(s): Tian X, Zhang A, Qiu C, Wang W, Yang Y, Qiu C, Liu A, Zhu L, Yuan S, Hu H, Wang W, Wei Q, Zhang X, Xu J. The upregulation of LAG-3 on T cells defines a subpopulation with functional exhaustion and correlates with disease progression in HIV-infected subjects. Journal of immunology (Baltimore, Md. : 1950) 2015 Apr 194 (8): 3873-82..PMID: 25780040