Goat anti-calbindin D28 Antibody

Goat anti-calbindin D28 Antibody
Principle Name: calbindin D28
Official Symbol: CALB1
All Name and Symbols: CALB1; calbindin 1, 28kDa; CALB; D-28K; OTTHUMP00000166027; OTTHUMP00000225441; RTVL-H protein; calbindin; calbindin 1, (28kD); calbindin D28; vitamin D-dependent calcium-binding protein, avian-type
Accession Number(s): NP_004920.1
Peptide Sequence: KTFVDQYGQRDDGK, is from internal region
Human Gene ID(s): 793
Non-Human GeneID(s): 83839 (rat);
Important Comments: Immunizing peptide overlaps EF hand 2, but not any calcium binding site.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB, IHC
Tested Species: Human, Mouse, Rat
Peptide ELISA: antibody detection limit dilution 1 to 128000.
Western Blot: Approx 26kDa band observed in Human Brain (Cerebellum) lysates and in Mouse and Rat total Brain lysates (calculated MW of 30.0kDa according to NP_004920.1). The observed molecular weight corresponds to earlier findings in literature with di
Immunohistochemistry: Frozen sections of Human Hypothalamus shows staining of Calbinding D2 neurons. Recommended concentration, 0.1-0.3ug/ml.
Reference(s): Kusakabe KT, Abe H, Kondo T, Kato K, Okada T, Otsuki Y. DNA microarray analysis in a mouse model for endometriosis and validation of candidate factors with human adenomyosis. J Reprod Immunol. 2010 Jun; 85(2):149-60..PMID: 20452033