Goat anti-BDNF Antibody

Goat anti-BDNF Antibody
Principle Name: BDNF
Official Symbol: BDNF
All Name and Symbols: BDNF ; brain-derived neurotrophic factor; MGC34632; neurotrophin
Accession Number(s): NP_001700.2; NP_001700.2; NP_733928.1; NP_733929.1; NP_733930.1; NP_733931.1
Peptide Sequence: ETKCNPMGYTKE, is from internal region
Human Gene ID(s): 627
Non-Human GeneID(s):
Important Comments: This antibody is expected to recognise all reported isoforms (NP_001700.2; NP_001700.2; NP_733928.1; NP_733929.1; NP_733930.1; NP_733931.1)
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, IHC, IF
Tested Species: Human, Mouse, Rat
Peptide ELISA: antibody detection limit dilution 1 to 4000.
Immunohistochemistry: In paraffin embedded Human Cortex shows cytoplasm staining of neuronal cells. Recommended concentration, 3-5ug/ml.
Frozen sections of Mouse sciatic nerve shows intensified staining after injury, consistent with the literature (Tonr
Reference(s): Yoshii A, Constantine-Paton M. BDNF induces transport of PSD-95 to dendrites through PI3K-AKT signaling after NMDA receptor activation. Nat Neurosci. 2007 Jun; 10(6):702-711. Epub 2007 May 21..PMID: 17515902