Anti-IgG2 (Fc subclass specific), TRITC conjugate

The reactivity of the antiserum is restricted to a subclass specific determinant on the Fc part of the IgG2 molecule as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunoperoxidase staining of cytoplasmic Ig. To identify the presence of IgG2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase staining of cytoplasmic IgG2, and immunoblotting using a peroxidase labelled monoclonal antibody against TRITC. The optimum working dilution is an assay-related characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:10 to 1:50, in ELISA from 1:200 upwards, in Western blotting from 1:400 upwards. Working dilutions may vary widely, strongly depending on the test conditions. These data should be interpreted as general recommendations only.

Tetramethylrhodamine isothiocyanate-conjugated purified monoclonal Mouse antibody to Human IgG2, subclass specific.

Highly purified monoclonal IgG2 isolated from human serum.

For Research Use Only.