Anti-IgG1 (Fc subclass specific), HRP conjugate

The reactivity of the antiserum is restricted to the subclass specific determinant on the Fc portion of the IgG1 molecule as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunoperoxidase staining of cytoplasmic Ig. To identify the presence of IgG1 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining of cytoplasmic IgG1, and immunoblotting. The optimum working dilution is an assay-related characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:10 to 1:40, in ELISA from 1:125 to 1:8, 000, in Western blotting from 1:250 to 1:10.000. These data should be interpreted as general recommendations only.

Peroxidase conjugated purified monoclonal Mouse antibody to Human IgG1, subclass specific.

Highly purified monoclonal IgG1 isolated from pooled human serum.

For Research Use Only.