Anti-IgG1 (Fc subclass specific)

The reactivity of the antiserum is restricted to a subclass specific determinant on the Fc part of the IgG1 molecule as tested in indirect binding enzyme immunoassay, immunoblotting, immunoprecipitation and indirect immunoperoxidase staining of cytoplasmic Ig To identify the presence of IgG1 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgG1, and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:500 upwards, in Western blotting from 1:1, 000 upwards. These data should be interpreted as general recommendations only.

Monoclonal Mouse antibody to Human IgG1, subclass specific.

Highly purified monoclonal IgG1 isolated from human serum

For Research Use Only.