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ExKine Nuclear and Cytoplasmic Protein Extraction Kit

ExKine Nuclear and Cytoplasmic Protein Extraction Kit

€546.00

Excl. VAT

Item no.	ABK-KTP3001-200T
Manufacturer	Abbkine Scientific
Amount	200T
Category	Other Assays & Kits Extraction Kits
Type	Assay-Kit
Specific against	other
ECLASS 10.1	32161090
ECLASS 11.0	32161090
UNSPSC	41116126
Available
Applications notes	Abbkine ExKine Nuclear and Cytoplasmic Protein Extraction Kit enable stepwise separation and preparation of crude cytoplasmic and nuclear extracts from mammalian cultured cells or tissue. This Kit is based on allowing cells to swell with hypotonic buffer. And then the cells are disrupted, the cytoplasmic fraction is removed, and the nuclear proteins are released from the nuclei by a high salt buffer. Non-denatured, active proteins are purified in less than two hours.
Kit components	Cytoplasmic Solution A (CES A) Cytoplasmic Solution B (CES B) Nuclear Extraction Solution (NES) DTT (500X) Protease Inhibitor (100X)
Features & Benefits	Versatile—suitable for fresh mammalian cultured cells and tissues with little or no cross-contaminations. Fast and convenient—non-denatured, active proteins are purified in less than two hours. Compatible—apply in various downstream assays, including Western blotting, gel-shift assays, protein assays, reporter gene assays and enzyme activity assays.
Usage notes	Perform all steps at 2–8 C. Use precooled buffers and equipment. Ensure all the solutions are defrosted and homogeneous.
Storage instructions	The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	The preparation of an extract from nuclei is often the first step in studying nuclear proteins and their interactions. The resulting preparation can be used directly in the Electrophoresis Mobility Shift Assay (EMSA), footprinting analysis, transcription assays, or as a starting point for the purification of regulatory proteins.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 200T

Listprice: €546.00

Price: €546.00

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Delivery expected until 9/18/2025

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