Mouse Cardiac troponin I (cTn-I) ELISA Kit

Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Troponin I is part of a heteromeric complex playing an important role in the regulation of skeletal and cardiac muscle contraction. It consists of three subunits, troponin I (TnI), troponin T (TnT) and troponin C (TnC). Each subunit is responsible for part of troponin complex function. TnI inhibits ATPase activity of acto myosin and TnT and TnI are present in cardiac muscles in different forms than in skeletal muscles. Only one tissue specific isoform of TnI is described for cardiac muscle tissue (cTnI) and this is expressed only in myocardium.Equally reactive with free cardiac troponin I (cTnI) and cTnI forming complexes with other troponin components. (In the presence of 5 mM EDTA). Antibodies are not affected by heparin, phosphorylation, oxidation and troponin complex formation. No cross-reaction with skeletal muscle troponin I.

This Mouse Cardiac troponin I (cTn-I) ELISA Kit employs a two-site sandwich ELISA to quantitate TNNI3.

Mouse Cardiac troponin I (cTn-I) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TNNI3. No significant cross-reactivity or interference between Mouse TNNI3 and analogues was observed.

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Cell culture supernatants#Serum#Plasma#Other biological fluids

TNNI3