Mouse Thyroid-Peroxidase (TPO) ELISA Kit

Colorimetric

Sandwich ELISA (quantitative)

Mouse Thyroid-Peroxidase microplate
Mouse Thyroid-Peroxidase standard
Mouse Thyroid-Peroxidase detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers

Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gel pack with blue ice.

Thrombopoietin (TPO), is a key regulator of megakaryocytopoiesis and thrombopoiesis in vitroand in vivo. TPO has been purified and cloned from several species including mouse, rat, and dog. The proteins from the various species are highly conserved, exhibiting from 69-75% sequence identity at the amino acid sequence level. Mature TPO can be divided into two domains: the amino-terminal half with homology to erythropoietin (Epo) and the carboxy-terminal half rich in serine, threonine and proline residues and containing seven potential N-linked glycosylation sites. The carboxy terminus domain of TPO has been shown to regulate the specific activity and circulating half-life of TPO. The carboxy-terminal may also have a role in promoting the efficient biosynthesis and secretion of TPO.

TPO; MSA; TDH2A; TPX; OTTHUMP00000115536; thyroid microsomal antigen; thyroperoxidase