Human Peptidoglycan recognition protein L (PGRP-L) ELISA Kit

Colorimetric

Sandwich ELISA (quantitative)

Human Peptidoglycan recognition protein L microplate
Human Peptidoglycan recognition protein L standard
Human Peptidoglycan recognition protein L detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers

Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gel pack with blue ice.

The deduced 576-amino acid precursor protein contains an N-terminal signal peptide, followed by 2 transmembrane segments and an extracellular C terminus containing PGRP domains III, II, and I. PGRPL shares 40%, 33%, and 32% identity with PGRPS, PGRPI-alpha, and PGRPI-beta, respectively. RNA dot blot analysis detected strong PGRPL expression in adult liver and weak expression in fetal liver. Northern blot analysis detected 2.1- and 0.8-kb transcripts expressed in adult and fetal liver. PCR also detected low expression in transverse colon, lymph nodes, heart, thymus, pancreas, descending colon, stomach, and testis. Transiently transfected COS-7 and human embryonic kidney cells expressed PGRPL as a Triton X-100-soluble membrane protein with an apparent molecular mass of 65 kD.

PGLYRP2; HMFT0141; PGLYRPL; PGRP-L; PGRPL; TAGL-like; tagL; tagL-alpha; tagl-beta; peptidoglycan recognition protein L; peptidoglycan recognition protein-like