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Human RAF proto-oncogene serine/threonine-protein kinase (RAF1) ELISA Kit

€22,389.00
Excl. VAT
Item no. ABK-KTE60981-96Tx50
Manufacturer Abbkine Scientific
Amount 96Tx50
Category
Type Elisa-Kit
Applications ELISA
Specific against Human (Homo sapiens)
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Available
Applications notes
This Human RAF proto-oncogene serine/threonine-protein kinase (RAF1) ELISA Kit employs a two-site sandwich ELISA to quantitate RAF1 in samples. An antibody specific for RAF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRAF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RAF1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RAF1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method
Colorimetric
Sample type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay type
Sandwich ELISA (quantitative)
Assay duration
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit components

Human RAF proto-oncogene serine/threonine-protein kinase microplate
Human RAF proto-oncogene serine/threonine-protein kinase standard
Human RAF proto-oncogene serine/threonine-protein kinase detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers
Features & Benefits
Human RAF proto-oncogene serine/threonine-protein kinase (RAF1) ELISA Kit has high sensitivity and excellent specificity for detection of Human RAF1. No significant cross-reactivity or interference between Human RAF1 and analogues was observed.
Usage notes
Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions
The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.
Shipping
Gel pack with blue ice.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background
RAF proto-oncogene serine/threonine-protein kinase (RAF1) is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in RAF1 are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
Gene ID
5894
Alternative
RAF1; CRAF; NS5; Raf-1; c-Raf; Oncogene RAF1; raf proto-oncogene serine/threonine protein kinase
Accession
P04049

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 96Tx50
Available: In stock
Listprice: €22,389.00
Price: €22,389.00
available

Delivery expected until 9/18/2025 

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