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Human Resistin-like Molecule beta (RELM-beta) ELISA Kit

Human Resistin-like Molecule beta (RELM-beta) ELISA Kit

€23,865.00

Excl. VAT

Item no.	ABK-KTE60803-96Tx50
Manufacturer	Abbkine Scientific
Amount	96Tx50
Category	Metabolism Diabetes Glucose Homeostasis Obesity ELISA ELISA & Immunoassays ELISA Kits Immunoassay
Type	Elisa-Kit
Applications	ELISA
Specific against	Human (Homo sapiens)
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Available
Applications notes	This Human Resistin-like Molecule beta (RELM-beta) ELISA Kit employs a two-site sandwich ELISA to quantitate RETNLB in samples. An antibody specific for RETNLB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRETNLB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RETNLB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RETNLB bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method	Colorimetric
Sample type	Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay type	Sandwich ELISA (quantitative)
Assay duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit components	Human Resistin-like Molecule beta microplate Human Resistin-like Molecule beta standard Human Resistin-like Molecule beta detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features & Benefits	Human Resistin-like Molecule beta (RELM-beta) ELISA Kit has high sensitivity and excellent specificity for detection of Human RETNLB. No significant cross-reactivity or interference between Human RETNLB and analogues was observed.
Usage notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions	The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	RELM-beta (Resistin-Like Molecule-beta) is a member of a recently identified family of secreted proteins containing a conserved cystein-rich C-terminus. The RELM family consists of resistin (also called FIZZ3), RELM-alpha (FIZZ1), RELM-beta (FIZZ2) and RELM-gamma. Only resisistin and RELM-beta were found in humans whereas all four RELM family members were identified in rodents. RELM-beta appears to be produced as a homodimer exclusively by intestinal goblet cells and can be found in high quantities in stool. Remarkably, stool of germ-free mice displaying sterile intestinal tract does not contain RELM-beta until bacterial colonization takes place after pathogen-free mice entered natural environment. Some, but not all, colon carcinoma cell lines secrete RELM-beta into the cell culture supernatant. The physiological function of RELM-beta is not known. High doses of recombinant RELM-beta showed hyperglycemic effects including lowered glucose disposal and increased hepatic glucose production in mice.
Gene ID	84666
Alternative	RETNLB; FIZZ1; FIZZ2; HXCP2; RELM-beta; RELMb; RELMbeta; XCP2; C/EBP-epsilon regulated myeloid-specific secreted cysteine-rich protein precursor 2; colon and small intestine-specific cysteine-rich protein; cy
Accession	Q9BQ08

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 96Tx50

Listprice: €23,865.00

Price: €23,865.00

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Delivery expected until 9/18/2025

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