Human Secretogranin-3 (SCG3) ELISA Kit

Colorimetric

Sandwich ELISA (quantitative)

Human Secretogranin-3 microplate
Human Secretogranin-3 standard
Human Secretogranin-3 detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers

Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gel pack with blue ice.

Secretogranin III is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. Granins may serve as precursors for biologically active peptides. Some granins have been shown to function as helper proteins in sorting and proteolytic processing of prohormones; however, the function of this protein is unknown.
The deduced 468-amino acid protein, which has a calculated molecular mass of 53 kD, contains an N-terminal signal peptide, a secretogranin motif, 3 potential N-glycosylation sites, and a putative tyrosine sulfation site. SCG3 shares 87%, 87%, and 56% overall identity with the mouse, rat, and Xenopus orthologs, respectively. SCG3 localized to the perinuclear endoplasmic reticulum in transfected COS-7 cells.

SCG3; FLJ90833; SGIII;