Comparison

Human Monocarboxylate transporter 1 (SLC16A1) ELISA Kit

€22,389.00
Excl. VAT
Item no. ABK-KTE60626-96Tx50
Manufacturer Abbkine Scientific
Amount 96Tx50
Category
Type Elisa-Kit
Applications ELISA
Specific against Human (Homo sapiens)
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Available
Applications notes
This Human Monocarboxylate transporter 1 (SLC16A1) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC16A1 in samples. An antibody specific for SLC16A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC16A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC16A1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC16A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method
Colorimetric
Sample type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay type
Sandwich ELISA (quantitative)
Assay duration
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit components

Human Monocarboxylate transporter 1 microplate
Human Monocarboxylate transporter 1 standard
Human Monocarboxylate transporter 1 detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers
Features & Benefits
Human Monocarboxylate transporter 1 (SLC16A1) ELISA Kit has high sensitivity and excellent specificity for detection of Human SLC16A1. No significant cross-reactivity or interference between Human SLC16A1 and analogues was observed.
Usage notes
Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions
The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.
Shipping
Gel pack with blue ice.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background
SLC16A1 mediates the movement of lactate and pyruvate across cell membranes Import and export of these substrates by tissues such as erythrocytes, muscle, intestine, and kidney are ascribed largely to the action of a proton-coupled MCT. The corresponding cDNA was isolated by an expression cloning strategy and found to encode a protein with 12 putative membrane-spanning regions. The cloned mutant 'mevalonate transporter' differed from its wildtype progenitor by 1 amino acid in the tenth membrane-spanning region, which changed a phenylalanine (wildtype) to a cysteine (mutant). The finding that the wildtype cDNA did not elicit increased mevalonate transport in transfected cells suggested that the wildtype protein is a transporter for a molecule other than mevalonate.
Gene ID
6566
Alternative
SLC16A1; FLJ36745; HHF7; MCT; MCT1; MGC44475; monocarboxylate transporter 1; solute carrier family 16 (monocarboxylic acid transporters); member 1; solute carrier family 16; member 1
Accession
P53985

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 96Tx50
Available: In stock
Listprice: €22,389.00
Price: €22,389.00
available

Delivery expected until 10/16/2025 

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