Anti-Cleaved-Caspase-9 (D353) antibody

Unconjugated

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Store at-20° C, and avoid repeat freeze-thaw cycles.

Caspase-9 is a protein encoded by the CASP9 gene which is approximately 46, 2 kDa. Caspase-9 is localised to the cytosol, mitochondrion and nucleus. It is involved in the TNFR1 pathway, RET signalling and apoptosis modulation and signalling. Caspase-9 exist as an inactive proenzyme which undergoes proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. It is involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Caspase-9 is ubiquitously expressed with highest expression in the heart. Mutations in the CASP9 gene may result in cerebral hypoxia and cervical cancer. STJ90011 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of fragment activated Caspase-9 protein resulting from cleavage adjacent to D353.

CASP9

280-360 aa, C-terminal

Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.

IF 1:50-200
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000

Caspase-9
CASP-9
Apoptotic protease Mch-6
Apoptotic protease-activating factor 3
APAF-3
ICE-like apoptotic protease 6
ICE-LAP6
Caspase-9 subunit p35
Caspase-9 subunit p10

P55211_HUMAN