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MERS-CoV Spike/S1 Protein (S1 Subunit, aa 1-725, His Tag)

MERS-CoV Spike/S1 Protein (S1 Subunit, aa 1-725, His Tag)

ArtNr	40069-V08H-1
Hersteller	Sino Biological
Menge	1mg
Kategorie	Infectious Diseases Viral Infections Coronavirus (COVID-19) Peptides & Proteins Recombinant Proteins
Typ	Proteins
Specific against	MERS-CoV
Host	Human
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	S
Similar products	Spike
Lieferbar
Molecule	Spike
Synonym	S
Storage	Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Protein Construction	A DNA sequence encoding the spike protein S1 (Human betacoronavirus 2c EMC/2012)( AFS88936.1)(Met1-Glu725) was fused with a polyhistidine tag at the C-terminus.
Expressed Host	Human Cells
Purity	> 95 % as determined by SDS-PAGE
Bio-Activity	1. Measured by its binding ability in a functional ELISA. Immobilized Spike Protein S1 (aa 1-725?(Cat: 40069-V08H) at 10 ug/ml (100 ul/well) can bind biotinylated human DPP4 (Cat: 10688-HNCH). The EC50 of of biotinylated DPP4 (Cat: 10688-HNCH) is 0.6-1.39 ug/ml. 2. Measured by its binding ability in a functional ELISA. Immobilized Spike Protein S1 (aa 1-725) (Cat: 40069-V08H) at 10 ug/ml (100 ul/well) can bind biotinylated Fc-DPP4 (Cat: 10688-H01H). The EC50 of biotinylated Fc-DPP4 (Cat: 10688-H01H) is 0.02-0.05 ug/ml.
Endotoxin	< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal	Tyr 18
Molecule Mass	The recombinant spike protein S1 (Human betacoronavirus 2c EMC/2012) comprises 719 amino acids and has a predicted molecular mass of 79.9 kDa. It migrates as an approximately 94 kDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% glycerol.
Accession	AFS88936.1
Amino acid sequence	Met1-Glu725
TAG	C-His

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Arbeitsanleitung (Data Sheet) anfordern

40069-V08H-1-datasheet.pdf

40069-V08H-1-safety-datasheet.pdf