pNPP Microwell Alkaline Phosphatase Stablized Substrate One Comp

pNPP Alkaline Phosphatase Microwell Substrate (p-nitrophenylphosphate) is a soluble substrate used with the enzyme alkaline phosphatase designed for various qualitative or quantitative immunoassays but not recommended for membrane or immunohistochemical applications where a precipitating reaction product is required. The substrate is supplied as a one component ready to use solution. Initially the substrate should be colorless to pale yellow in appearance. When this substrate system is reacted with alkaline phosphatase, a soluble, yellow product is obtained.

Store at temperatures between 2-8ºC. The substrate performance has been shown to be within specifications following 5 freeze-thaw cycles, however freezing is not recommended. The substrate should be protected from direct light by storing in amber bottles. Only high quality glass and plastic products should be used for storing aliquots.