Mouse VEGF ELISA

RayBio Mouse VEGF-A ELISA Kit for serum, plasma, cell culture supernatants

96-well Microplate

Quantitative

Cell Culture Supernatants, Plasma, Serum

2 pg/ml - 1000 pg/ml

This ELISA kit shows no cross-reactivity with any of the cytokines tested (e.g., Mouse 6Ckine, CTACK, Eotaxin, GCSF, GM-CSF, IL-2, IL-3, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12p40, IL-12p70, IL-13, IL-17, IFN-gamma, KC, Leptin, MCP-5, MIP-1alpha, MIP-2, MIP-3beta, RANTES, SCF, sTNFri, TARC, TIMP-1, TNF-alpha, Tpo).

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 ul of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4C.
4. Add 100 ul of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 ul of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 ul of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 ul of Stop Solution to each well.
11. Read at 450 nm immediately.

1. Saldaña L, Crespo L, Bensiamar F, et al. Mechanical forces regulate stem cell response to surface topography. J Biomed Mater Res A. 2013 Apr 24, [Epub ahead of print] doi:10.1002/jbm.a.34674.
2. Said N, Frierson HF, Sanchez-Carbayo M, Brekken RA, Theodorescu D. Loss of SPARC in bladder cancer enhances carcinogenesis and progression. J Clin Invest. 2013 February 1, 123(2): 751–766.
3. Xiao X, Guo P, Chen Z, et al. Hypoglycemia reduces vascular endothelial growth factor A production by pancreatic beta cells as a regulator of beta cell mass. J Biol Chem. 2013 Mar 22, 288(12):8636–8646.
4. Martin D, Nguyen Q, Molinolo A, Gutkind JS. Accumulation of dephosphorylated 4EBP after mTOR inhibition with rapamycin is sufficient to disrupt paracrine transformation by the KSHV vGPCR oncogene. Oncogene. 2013 May 27, [Epub ahead of print] doi: 10.1038/onc.2013.193.
5. Hartwich J, Orr WS, Ng CY, et al. HIF-1alpha activation mediates resistance to anti-angiogenic therapy in neuroblastoma xenografts. J. Pediatr. Surg. 2013 Jan, 48(1):39–46.
6. Hartwich J, Orr WS, Ng CY, et al. HIF-1alpha activation mediates resistance to anti-angiogenic therapy in neuroblastoma xenografts. J. Pediatr. Surg. 2013 Jan, 48(1):39–46.
7. Bhuvaneswari R, Gan Y, Yee K, Soo K, Olivo M. Effect of hypericin-mediated photodynamic therapy on the expression of vascular endothelial growth factor in human nasopharyngeal carcinoma. Int J Mol Med. 2007, 20:421-428.
8. Nasr M., Nafee N., Saad H., Kazem A. Improved antitumor activity and reduced cardiotoxicity of epirubicin using hepatocyte-targeted nanoparticles combined with tocotrienols against hepatocellular carcinoma in mice. European Journal of Pharmaceutics and Biopharmaceutics. Available online 6 May 2014, http://dx.doi.org.proxy.library.emory.edu/10.1016/j.ejpb.2014.04.016 [Epub ahead of print]
9. Hartwich J., Orr S., Ng C., Spence Y., Morton C., DavidoffA. HIF-1[alpha] activation mediates resistance to anti-angiogenic therapy in neuroblastoma xenografts. Journal of Pediatric Surgery Volume 48, Issue 1 , Pages 39-46, January 2013
10. Konac E., et al. Does Pattern Scan Laser (PASCAL) photocoagulation really induce less VEGF expression in murine retina than conventional laser treatment? Gene. In Press, Corrected Proof, Available online 24 July 2014. http://dx.doi.org/10.1016/j.gene.2014.07.062
11. Abbas OL., et al. Inhibition of the Notch Pathway Promotes Flap Survival by Inducing Functional Neoangiogenesis. Annals of Plastic Surgery August 28, 2014, epub ahead of print. doi: 10.1097/SAP.0000000000000197
12. Wei, Z., Yu, S. et al. Regulatory Role of the JNK-STAT1/3 Signaling in Neuronal Differentiation of Cultured Mouse Embryonic Stem Cells. Cellular and Molecular Neurobiology 2014. Epub Ahead of Print: DOI: 10.1007/s10571-014-0067-4