Human VCAM-1 ELISA

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ArtNr	ELH-VCAM1-2
Hersteller	Raybiotech
Menge	2 x 96-Well Strip Microplate Kit
Kategorie	Oncology Neuroscience Neuroinflammation Cell Biology Cell Movement Cell Adhesion ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Renal Cancer
Typ	Elisa-Kit
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
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Short description	RayBio Human VCAM-1 ELISA Kit for serum, plasma, cell culture supernatants, and urine
Target Name and Synonyms	CD106, VCAM-1
Solid Support	96-well Microplate
Design Principle	Sandwich-based
Result Output	Quantitative
Method of Detection	Colorimetric
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum, Urine
Sensitivity	300 pg/ml
Detection Range	0.3 ng/ml - 60 ng/ml
Recommended Dilution (serum & plasma)	10-50 fold
Specificity	This ELISA kit shows no cross-reactivity with any of the cytokines tested (e.g., human Angiopoietin-1, Angiostatin , B7-1, BMP-7, CD14, CD30, CD40, CD40 Ligand, CTLA-4, CXCL16, Dkk-4 , DR6, Endostatin, E-Selectin, Follistatin, HB-EGF, HVEM, ICAM-2, IGF-II, IL-10 Ra, IL-10 Rb, IL-18, IL-9 , IL-2 Ra, IL-2 Rb, IL-5 Ra, LAP, L-Selectin, MCSF R, MMP-1, 2, 3, 7, 8, 9, 10, 12 and 13, PDGF-AB, SDF-1b, Tie-1, Tie- 2, TIMP-3.
Product Features	Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Kit Components	Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Other Materials Required	Distilled or deionized water Precision pipettes to deliver 2 ul to 1 ul volumes Adjustable 1-25 ul pipettes for reagent preparation 100 ul and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
Protocol Summary	Prepare all reagents, samples and standards as instructed in the manual. Add 100 ul of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4C. Add 100 ul of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 ul of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 ul of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 ul of Stop Solution to each well. Read at 450 nm immediately.
Storage	May be stored for up to 6 months at 2C to 8C from the date of shipment. Standard (recombinant protein) should be stored at -20C or -80C (recommended at -80C) after reconstitution. Opened Microplate Wells or reagents may be store for up to 1 month at 2C to 8C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: the kit can be used within one year if the whole kit is stored at -20C.
Citation	1. Comparison of gene expression profile of epiretinal membranes obtained from eyes with proliferative vitreoretinopathy to that of secondary epiretinal membranes 2. Fadel F., Elshamaa M., Nabhan M., Essam R., Kantoush N., et al. Soluble adhesion molecules as markers of native arteriovenous fistula thrombosis in children on uremia. Blood Coagulation and Firbrinolysis. Published online DOI:10.1097/MBC.0000000000000125 [Epub ahead of Print] 3. Wadood S., Shawk R., Baher H. Intercellular Adhesion Molecule-1, Vascular Adhesion Molecule-1, Interlukin1-beta, and C - reactive protein Levels in Iraqi Patients with Type 2 Diabetes Mellitus. IOSR J of Dental and Medical SciencesVolume 13, Issue 4 Ver. IV. (Apr. 2014), PP55-62

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