Human TNF-alpha ELISA

RayBio Human TNF-alpha ELISA Kit for serum, plasma, cell culture supernatants, and urine

96-well Microplate

Quantitative

Cell Culture Supernatants, Plasma, Serum, Urine

30 pg/ml - 6000 pg/ml

This ELISA kit shows no cross-reactivity with any of the cytokines tested (e.g., human ANG, CD23, Eotaxin, GCSF, GM-CSF, GRO- alpha, GRO-beta, GRO-gamma, I-309, IFN-gamma, IL-1alpha, IL-1beta, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p40), IL-12 (p70), IL-15, IL-16, IP-10, MCP-1, MCP-2, MCP-3, MCP-4, MCSF, MIG, MIP-1alpha, MIP-1beta, NAP-2, PDGF, PF-4,

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 ul of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4C.
4. Add 100 ul of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 ul of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 ul of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 ul of Stop Solution to each well.
11. Read at 450 nm immediately.

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