Human IL-8 ELISA

RayBio Human IL-8 ELISA Kit for serum, plasma, cell culture supernatants, and urine

96-well Microplate

Quantitative

Cell Culture Supernatants, Plasma, Serum, Urine

1 pg/ml - 600 pg/ml

This ELISA kit shows no cross-reactivity with any of the cytokines tested (e.g., human BDNF, BLC, ENA-78, FGF-4, IL-1alpha, IL-1beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1alpha, MIP-1 beta, MIP-1gamma, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF).

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 ul of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4C.
4. Add 100 ul of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 ul of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 ul of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 ul of Stop Solution to each well.
11. Read at 450 nm immediately.

1. Wu S-J, No L-T. Tetrandrine Inhibits Proinflammatory Cytokines, iNOS and COX-2 Expression in Human Monocytic Cells. Biol Pharm Bull. 2007, 30:59–62.
2. Ertugrul AS, Sahin H, Dikilitas A, et al. Comparison of CCL28, interleukin-8, interleukin-1beta and tumor necrosis factor-alpha in subjects with gingivitis, chronic periodontitis and generalized aggressive periodontitis. J Periodont Res. 2013 Feb, 48(1):44–51.
3. Martins AB. Serum myeloperoxidase level is increased in heavy smokers. Open J Clin Diagn. 2013, 03(01):5–8.
4. Sobrino, F., Maria E. Reyes-Quiroz, and Javier Saenz. "Platelet-activating Factor Downregulates the Expression of Liver X Receptor-alpha and Its Target Genes in Human Neutrophils." The FEBS Journal. Wiley Online Library, 02 Jan. 2014. Web.
5. Lo MC., et al. Role of MIF/CXCL8/CXCR2 signaling in the growth of nasopharyngeal carcinoma tumor spheres. Cancer Lett. 2013 Jul 10, 335(1):81-92. doi: 10.1016/j.canlet.2013.01.052.
6. Guglielmetti S, Tamagnini I, Mora D, Minuzzo M , Scarafoni A, et al.Implication of an Outer Surface Lipoprotein in Adhesion of Bifidobacterium bifidum to Caco-2 Cells. Appl Environ Microbiol. 2008:74(15):4695–4702.
7. Capo X., Mortorell M., Llompart I., Sureda A., Tur J., Pons A., Docosahexanoic acid diet supplementation attenuates the peripheral mononuclear cell inflammatory response to exercise following LPS activation. Cytokine 69 (2014) 155–164 DOI:10.1016/j.cyto.2014.05.026
8. Miethiling-Graff R., et al. Exposure to silver nanoparticles induces size- and dose-dependent oxidative stress and cytotoxicity in human colon carcinoma cells. Toxicology in vitro. Available online 2 July 2014. DOI: 10.1016/j.tiv.2014.06.005
9. Koob T., et al. Cytokines in single layer amnion allografts compared to multilayer amnion/chorion allografts for wound healing. Journal of Biomedical Materials Research Part B: Applied Biomaterials. Aug 2014, epub ahead of print. DOI: 10.1002/jbm.b.33265
10. Sanmamed M., et al. Serum interleukin-8 reflects tumor burden and treatment response across malignancies of multiple tissue origins. Clinical Cancer Research, Sept 2014, epub ahead of print.
11. Shaker MJ., et al. Immunological Study of Women Infected with Trichomonas vaginalis Parasite in Baghdad city. International Journal of Science and Research. Volume 3 Issue 9, September 2014,
12. Lu H., et al. A breast cancer stem cell niche supported by juxtacrine signalling from monocytes and macrophages. Nature Cell Biology (2014) doi:10.1038/ncb3041