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Mouse Cytokine Array C1

ArtNr AAM-CYT-1000-8
Hersteller Raybiotech
Menge 8 Sample Kit
Kategorie
Typ Assay
ECLASS 10.1 32161000
ECLASS 11.0 32161000
UNSPSC 41116126
Lieferbar
Short description
RayBio C-Series Mouse Cytokine Antibody Array 1000. A combination of Mouse Cytokine Antibody Array C3 & C4. Detects 96 Mouse Cytokines. Suitable for all liquid sample types.
Features
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
Number of Targets Detected
96
Target Names
Axl, bFGF, BLC (CXCL13), CD30 Ligand (TNFSF8), CD30 (TNFRSF8), CD40 (TNFRSF5), CRG-2, CTACK (CCL27), CXCL16, CD26 (DPPIV), Dtk, Eotaxin-1 (CCL11), Eotaxin-2 (MPIF-2/CCL24), E-Selectin, Fas Ligand (TNFSF6), Fc gamma RIIB (CD32b), Flt-3 Ligand, Fractalkine (CX3CL1), GCSF, GITR (TNFRSF18), GM-CSF, HGFR, ICAM-1 (CD54), IFN-gamma, IGFBP-2, IGFBP-3, IGFBP-5, IGFBP-6, IGF-1, IGF-2, IL-1 beta (IL-1 F2), IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-15, IL-17A, IL-17 RB, IL-1 alpha (IL-1 F1), IL-2, IL-3, IL-3 R beta, IL-4, IL-5, IL-6, IL-7, IL-9, I-TAC (CXCL11), KC (CXCL1), Leptin, Leptin R, LIX, L-Selectin (CD62L), Lungkine (CXCL15), Lymphotactin (XCL1), MCP-1 (CCL2), MCP-5, M-CSF, MDC (CCL22), MIG (CXCL9), MIP-1 alpha (CCL3), MIP-1 gamma, MIP-2, MIP-3 beta (CCL19), MIP-3 alpha (CCL20), MMP-2, MMP-3, Osteopontin (SPP1), Osteoprotegerin (TNFRSF11B), Platelet Factor 4 (CXCL4), Pro-MMP-9, P-Selectin, RANTES (CCL5), Resistin, SCF, SDF-1 alpha (CXCL12 alpha), Sonic Hedgehog N-Terminal (Shh-N), TNF RI (TNFRSF1A), TNF RII (TNFRSF1B), TARC (CCL17), I-309 (TCA-3/CCL1), TECK (CCL25), TCK-1 (CXCL7), TIMP-1, TIMP-2, TNF alpha, Thrombopoietin (TPO), TRANCE (TNFSF11), TROY (TNFRSF19), TSLP, VCAM-1 (CD106), VEGF-A, VEGFR1, VEGFR2, VEGFR3, VEGF-D
Targets Detected
AXL, bFGF, BLC, CCL1, CCL11, CCL17, CCL19, CCL2, CCL20, CCL22, CCL24, CCL25, CCL27, CCL3, CCL5, CD106, CD26, CD30, CD30 Ligand, CD32b, CD40, CD54, CD62L, CRG-2, CTACK, CX3CL1, CXCL1, CXCL11, CXCL12 alpha, CXCL13, CXCL14, CXCL15, CXCL16, CXCL4, CXCL7, CXCL9, DPPIV, Dtk, E-Selectin, Eotaxin-1, Eotaxin-2, Fas Ligand, Fc gamma RIIB, Flt-3 Ligand, Fractalkine, G-CSF, GITR, GM-CSF, HGFR, I-309, I-TAC, ICAM-1, IFN-gamma, IGF-1, IGF-2, IGFBP-2, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-1 F1, IL-1 F2, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-15, IL-17, IL-17B R, IL-2, IL-3, IL-3 R beta, IL-4, IL-5, IL-6, IL-7, IL-9, KC, L-Selectin, Leptin, Leptin R, LIX, Lungkine, Lymphotactin, M-CSF, MCP-1, MCP-5, MDC, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 alpha, MIP-3 beta, MMP-2, MMP-3, MPIF-2, Osteopontin, Osteoprotegerin, P-Selectin, Platelet Factor 4, Pro-MMP-9, RANTES, Resistin, SCF, SDF-1 alpha, Shh-N, Sonic Hedgehog N-Terminal, SPP1, TARC, TCA-3, TCK-1, TECK, Thrombopoietin, TIMP-1, TIMP-2, TNF alpha, TNFRSF11B, TNFRSF18, TNFRSF19, TNFRSF5, TNFRSF8, TNFSF11, TNFSF6, TNFSF8, TPO, TRANCE, TROY, TSLP, VCAM-1, VEGF-A, VEGF-D, VEGFR1, VEGFR2, VEGFR3, XCL1
Suggested Applications
Multiplexed Protein Detection, Detection of Relative Protein Expression, Detecting Patterns of Cytokine Expression, Biomarker/ Key Factor Screening, Identifying Key Factors, Confirming a Biological Process
Kit Components
  • Mouse Cytokine Antibody Array C1000 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVPs ChemiDoc-It or EpiChem II Benchtop Darkroom), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis
Storage
For best results, store the entire kit frozen at -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20C and all other reagents undiluted at 4C for no more than 3 months.
Solid Support
Membrane
Design Principle
Sandwich-based
Result Output
Semi-Quantitative
Detection Method
Chemiluminescence
Compatible Sample Types
Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Citation
1. Cyclophilin D Extramitochondrial Signaling Controls Cell Cycle Progression and Chemokine-Directed Cell Motility
2. Masaki T., Qu J., Cholewa-Waclaw J., Burr K., Raaum R., Rambukkan A., Reprogramming Adult Schwann Cells to Stem Cell-like Cells by Leprosy Bacilli Promotes Dissemination of Infection. Cell Volume 152, Issues 1-2, p51–67, 17 January 2013
3. Braig, M., Pallman N., Preukschas M., Steinemann D., Hofmann W., Gompf A., et al. A 'telomere-associated secretory phenotype' cooperates with BCR-ABL to drive malignant proliferation of leukemic cells. Leukemia 1 April 2014, doi: 10.1038/leu.2014.95 [Epub Ahead of Print]
4. Barzelay A., Ben-Shoshan J., Entin-Meer M., Maysel-Auslender S., et al. A potential role for islet-1 in post-natal angiogenesis and vasculogenesis. Thromb Haemost 2010, 103: 188–197
5. Palmi C., et al. Cytoskeletal Regulatory Gene Expression and Migratory Properties of B Cell Progenitors are Affected by the ETV6-RUNX1 Rearrangement Mol Cancer Res molcanres.0056.2014, Published OnlineFirst July 24, 2014, doi: 10.1158/1541-7786.MCR-14-0056-T
6. Stanford J. Efferocytosis produces a prometastatic landscape during postpartum mammary gland involution. JCI Sept 2014, epub ahead of print. doi:10.1172/JCI76375.
7. Novitskiy et al.: Attenuated transforming growth factor beta signaling promotes metastasis in a model of HER2 mammary carcinogenesis. Breast Cancer Research 2014 16:425.\

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 8 Sample Kit
Lieferbar: In stock
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Lieferung vsl. bis 03.07.2025 

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